Journal: The Journal of Cell Biology

Article Title: Ectodomain shedding of L1 adhesion molecule promotes cell migration by autocrine binding to integrins

doi: 10.1083/jcb.200101099

Figure Lengend Snippet: L1 is shed without cytoplasmic tail. (A) CHO or L1-transfected CHO cells were incubated at 37°C in suspension in medium without FCS for 1 h. The cell pellet was lysed and analyzed by Western blot with mAb UJ127.11 to the ectodomain or pcytL1 to the cytoplasmic domain of L1. (B) Schematic representation of L1 cleavage fragments and their relative localization. The location of antibody recognition sites are indicated. (1), mAb 5G3; (2), mAb UJ 127.11; (3) pcytL1.

Article Snippet: The mAb to αvβ5 (P1F6) was obtained from Chemicon, the mAb to human CD49e (SAM-1) was from Dianova, the human L1 mAb 5G3 was obtained from Becton Dickinson, and the mAb to CD49f (GoH3) was obtained from Beckman Coulter.

Techniques: Transfection, Incubation, Suspension, Western Blot

Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

Article Title: Alterations in PD-L1 Expression Associated with Acquisition of Resistance to ALK Inhibitors in ALK -Rearranged Lung Cancer

doi: 10.4143/crt.2018.486

Figure Lengend Snippet: Programmed cell death–ligand 1 (PD-L1) protein levels in the parental H3122 and its subclonal cell lines with single- and double-resistant cell lines by Western blot (band intensities were measured by densitometry using Image J and normalized to those of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) serving as a loading control) (A) and fluorescence-activated cell sorting analysis. Histograms depict PD-L1 expression (B). PD-L1 mRNA levels. The error bars indicate the standard deviation (C).

Article Snippet: Cells were resuspended in 100 μL FACS buffer and then stained with mouse anti-human PD-L1 (MIH1, BD Pharmingen, San Jose, CA) or a mouse IgG1 isotype control for 30 minutes on ice in staining buffer (2% bovine serum albumin and 0.01% sodium azide).

Techniques: Western Blot, Fluorescence, FACS, Expressing, Standard Deviation

Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

Article Title: Alterations in PD-L1 Expression Associated with Acquisition of Resistance to ALK Inhibitors in ALK -Rearranged Lung Cancer

doi: 10.4143/crt.2018.486

Figure Lengend Snippet: Representative images of programmed cell death–ligand 1 (PD-L1) immunohistochemistry in non-small cell lung cancer patients (×400). PD-L1 score was graded as absent (score 0) (A), weak (score 1) (B), moderate (score 2) (C), or strong (score 3) (D). A score of 2 or 3 was deemed positive for PD-L1 expression.

Article Snippet: Cells were resuspended in 100 μL FACS buffer and then stained with mouse anti-human PD-L1 (MIH1, BD Pharmingen, San Jose, CA) or a mouse IgG1 isotype control for 30 minutes on ice in staining buffer (2% bovine serum albumin and 0.01% sodium azide).

Techniques: Immunohistochemistry, Expressing

Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

Article Title: Alterations in PD-L1 Expression Associated with Acquisition of Resistance to ALK Inhibitors in ALK -Rearranged Lung Cancer

doi: 10.4143/crt.2018.486

Figure Lengend Snippet: Changes in programmed cell death–ligand 1 (PD-L1) before and after crizotinib treatment in anaplastic lymphoma kinase–positive non-small cell lung cancer tumor tissues based on H-score (A), PD-L1 score (0, 1, 2, 3) (B), and positivity (C).

Article Snippet: Cells were resuspended in 100 μL FACS buffer and then stained with mouse anti-human PD-L1 (MIH1, BD Pharmingen, San Jose, CA) or a mouse IgG1 isotype control for 30 minutes on ice in staining buffer (2% bovine serum albumin and 0.01% sodium azide).

Techniques:

Journal: Kidney international

Article Title: The L1 cell adhesion molecule is a potential biomarker of human distal nephron injury in acute tubular necrosis.

doi: 10.1038/sj.ki.5002640

Figure Lengend Snippet: Figure 4 | Comparative localization of L1 intra- and extracytoplasmic domains. (a) L1 scheme showing the extracellular part with six immunoglobulin-like domains (Ig-like) (including one recognized by mAb272) and five fibronectin III-like (FNIII) domains followed by the transmembrane segment and cytoplasmic fragments (recognized by the goat polyclonal serum). (b–d) Normal CDs are compared with (e–g) damaged CDs. (b, e) CDs are stained by goat polyclonal antiserum labeled by FITC (green) and (c, f) mAb272 labeled by rhodamine (red). (d, g) Merge. (d) In normal CD, both antibodies give a basolateral signal. (g) In injured principal cells, the basolateral signal is low with both antibodies, whereas there is a strong apical membrane (arrow) signal with the anti-cytoplasmic domain antibody contrasting with no signal for the extracellular fragment. Original magnification 630.

Article Snippet: After four washes with TBS-T (Tris-buffered saline with 0.1% Tween), goat anti-human L1 extracellular domain antibody was added (R&D Systems), followed by anti-goat coupled to alkaline phosphatase (Sigma-Aldrich, St Louis, MO, USA).

Techniques: Staining, Labeling, Membrane

Journal: Investigative and Clinical Urology

Article Title: Improving the diagnosis of high grade and stage bladder cancer by detecting increased urinary calprotectin expression in tumor tissue and tumor-associated inflammatory response

doi: 10.4111/icu.2019.60.5.343

Figure Lengend Snippet: Comparison of urinary and tissue calprotectin levels of patient and control groups

Article Snippet: The level of urinary calprotectin was measured by the enzyme-linked immunosorbent assay (ELISA) method (Human Calprotectin ELISA kit; MyBiosource, San Diego, CA, USA) after centrifuging the urine samples at 3,000 rpm for 20 minutes.

Techniques: Comparison, Control

Journal: Investigative and Clinical Urology

Article Title: Improving the diagnosis of high grade and stage bladder cancer by detecting increased urinary calprotectin expression in tumor tissue and tumor-associated inflammatory response

doi: 10.4111/icu.2019.60.5.343

Figure Lengend Snippet: Comparison of urinary and tissue calprotectin levels of patient and control groups (female patients excluded)

Article Snippet: The level of urinary calprotectin was measured by the enzyme-linked immunosorbent assay (ELISA) method (Human Calprotectin ELISA kit; MyBiosource, San Diego, CA, USA) after centrifuging the urine samples at 3,000 rpm for 20 minutes.

Techniques: Comparison, Control

Journal: Investigative and Clinical Urology

Article Title: Improving the diagnosis of high grade and stage bladder cancer by detecting increased urinary calprotectin expression in tumor tissue and tumor-associated inflammatory response

doi: 10.4111/icu.2019.60.5.343

Figure Lengend Snippet: Urinary and tissue calprotectin levels of papillary, solid, and mixed tumors

Article Snippet: The level of urinary calprotectin was measured by the enzyme-linked immunosorbent assay (ELISA) method (Human Calprotectin ELISA kit; MyBiosource, San Diego, CA, USA) after centrifuging the urine samples at 3,000 rpm for 20 minutes.

Techniques:

Journal: Investigative and Clinical Urology

Article Title: Improving the diagnosis of high grade and stage bladder cancer by detecting increased urinary calprotectin expression in tumor tissue and tumor-associated inflammatory response

doi: 10.4111/icu.2019.60.5.343

Figure Lengend Snippet: Comparison of tumor grade and urinary and tissue calprotectin levels

Article Snippet: The level of urinary calprotectin was measured by the enzyme-linked immunosorbent assay (ELISA) method (Human Calprotectin ELISA kit; MyBiosource, San Diego, CA, USA) after centrifuging the urine samples at 3,000 rpm for 20 minutes.

Techniques: Comparison

Journal: Investigative and Clinical Urology

Article Title: Improving the diagnosis of high grade and stage bladder cancer by detecting increased urinary calprotectin expression in tumor tissue and tumor-associated inflammatory response

doi: 10.4111/icu.2019.60.5.343

Figure Lengend Snippet: Comparison of tumor stage with urinary and tissue calprotectin levels

Article Snippet: The level of urinary calprotectin was measured by the enzyme-linked immunosorbent assay (ELISA) method (Human Calprotectin ELISA kit; MyBiosource, San Diego, CA, USA) after centrifuging the urine samples at 3,000 rpm for 20 minutes.

Techniques: Comparison

Journal: Investigative and Clinical Urology

Article Title: Improving the diagnosis of high grade and stage bladder cancer by detecting increased urinary calprotectin expression in tumor tissue and tumor-associated inflammatory response

doi: 10.4111/icu.2019.60.5.343

Figure Lengend Snippet: Receiver operating characteristic (ROC) curve analysis of urinary and tissue calprotectin levels in the diagnosis of primary bladder cancer (area under the curve [AUC]=0.976, 95% confidence interval [CI], 0.929–1.023, p<0.0001; AUC=0.713, 95% CI, 0.579–0.847; respectively, for urinary and tissue calprotectin levels).

Article Snippet: The level of urinary calprotectin was measured by the enzyme-linked immunosorbent assay (ELISA) method (Human Calprotectin ELISA kit; MyBiosource, San Diego, CA, USA) after centrifuging the urine samples at 3,000 rpm for 20 minutes.

Techniques: Biomarker Discovery

Journal: Investigative and Clinical Urology

Article Title: Improving the diagnosis of high grade and stage bladder cancer by detecting increased urinary calprotectin expression in tumor tissue and tumor-associated inflammatory response

doi: 10.4111/icu.2019.60.5.343

Figure Lengend Snippet: Receiver operating characteristic (ROC) curve analyses of urinary calprotectin level in the discrimination of high-grade from lower grade tumors and non-muscle-invasive from muscle-invasive tumors (area under the curve [AUC]=0.92, 95% confidence interval [CI], 0.671–0.913, p<0.0001; and AUC=0.85, 95% CI, 0.69–1.00, p=0.01; respectively).

Article Snippet: The level of urinary calprotectin was measured by the enzyme-linked immunosorbent assay (ELISA) method (Human Calprotectin ELISA kit; MyBiosource, San Diego, CA, USA) after centrifuging the urine samples at 3,000 rpm for 20 minutes.

Techniques: